Oligonucleotide Ordering Form

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Synthesizability
Estimated Minimum Yield (nmol)*
%GC Content
Total Bases
Modifications
Molecular Mass (Da)
Estimated E260 (M-1cm-1)
Estimated Tm (°C)*
Sequence Assembly
*Notes
  • Estimated minimum yield is not guaranteed.
  • Tm calculation is performed with the following conditions: 50 mM Na+,
    3 mM Mg++, 1 µM oligonucleotide (equimolar to complement).

Optional Services

  • Probe minimum – 25 nmols
  • Primer minimum – 100 nmols

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- The use of oligonucleotides modified with 3’-MGB-Quenchers and 5’-Fluorophores is limited to human IVD applications only.
- ELITechGroup MDx holds patents on MGB, DSQ, AquaPhluor dyes, Super Bases, and oligonucleotide applications. Research-scale oligonucleotides containing these technologies are available for use without a license for research applications. Licensing may be required for large-scale orders and non-research use. Contact ELITechGroup MDx for more information.

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Fluorophores

name description abbreviation part number
Epoch Blue 380 Internal Phosphoramidite fluorophore, 381/460 nm [EB380-IP] M830192
Epoch Blue 380-HEG Phosphoramidite fluorophore, 381/460 nm, long linker [EB380-HEG-P] M830727
Epoch Blue 380 Polystyrene fluorophore, 381/460 nm [EB380-SS] M100441
6-FAM Phosphoramidite fluorophore, 496/517 nm, short linker [FAM-P] M100011
FAM-HEG Phosphoramidite fluorophore, 496/517 nm, long linker [FAM-HEG-IP] M830100
FAM-HEG Polystyrene fluorophore, 496/517 nm, long linker [FAM-HEG-SS] M100442
FAM-dU 3'-Phosphoramidite uridine-fluorescein conjugate, 496/517 nm, standard [FAM-dU-3'-P] M830763
FAM-dU 5'-Phosphoramidite uridine-fluorescein conjugate, 496/517 nm, reverse [FAM-dU-5'-P] M830764
FAM-Super I 3'-Phosphoramidite Super I conjugated with fluorescein, 496/517 nm, standard [FAM-SuperI-3'-P] M830783
Gig Harbor Green Polystyrene fluorophore, 497/521 nm, short linker [GG-SS] M100433
AquaPhluor 525 Internal Phosphoramidite fluorophore, 527/549 nm, short linker [AP525-IP] M830199
AquaPhluor 525 Terminal Phosphoramidite fluorophore, 527/549 nm, short linker [AP525-TP] M820714
AquaPhluor 525-HEG Phosphoramidite fluorophore, 527/549 nm, long linker [AP525-HEG-P] M100104
AquaPhluor 525 Polystyrene fluorophore, 527/549 nm, short linker [AP525-SS] M100276
Yakima Yellow Phosphoramidite fluorophore, 525/547 nm, short linker [YY-P] M830552
Yakima Yellow Polystyrene fluorophore, 525/547 nm, short linker [YY-SS] M100443
AquaPhluor 530 Terminal Phosphoramidite fluorophore, 531/552 nm, short linker [AP530-TP] M830803
AquaPhluor 559 Internal Phosphoramidite fluorophore, 559/581 nm, long linker [AP559-IP] M830195
AquaPhluor 559 Terminal Phosphoramidite fluorophore, 559/581 nm, short linker [AP559-TP] M830698
AquaPhluor 559 Polystyrene fluorophore, 559/581 nm, short linker [AP559-SS] M100278
AquaPhluor 570 Terminal Phosphoramidite fluorophore, 570/592 nm, short linker [AP570-TP] M830699
AquaPhluor 570 Internal Phosphoramidite fluorophore, 570/592 nm, short linker [AP570-IP] M830675
AquaPhluor 570 Polystyrene fluorophore, 570/592 nm, short linker [AP570-SS] M100444
Redmond Red Phosphoramidite fluorophore, 580/594 nm, short linker [RR-P] M830035
Redmond Red Polystyrene fluorophore, 580/594 nm, short linker [RR-SS] M100445
AquaPhluor 593 Terminal Phosphoramidite fluorophore, 593/613 nm, short linker [AP593-TP] M830736
AquaPhluor 593 Polystyrene fluorophore, 593/613 nm, short linker [AP593-SS] M100257
AquaPhluor 639 Terminal Phosphoramidite fluorophore, 593/613 nm, short linker [AP639-TP] M830741
AquaPhluor 639 Polystyrene fluorophore, 639/655 nm, short linker [AP639-SS] M100440
AquaPhluor 642 Polystyrene fluorophore, 641/653 nm, short linker [AP642-SS] M100280
AquaPhluor 662 Polystyrene fluorophore, 662/672 nm, short linker [AP662-SS] M100271
AquaPhluor 680 Polystyrene fluorophore, 682/704 nm, short linker [AP680-SS] M100282
AquaPhluor 690 Terminal Phosphoramidite fluorophore, 689/709 nm, short linker [AP690-TP] M830760
AquaPhluor 690 Polystyrene fluorophore, 689/709 nm, short linker [AP690-SS] M100500
Quasar 705 Terminal Phosphoramidite fluorophore, 690/705 nm [QSR705-TP] M100274
Cyanine 5.5 Terminal Phosphoramidite fluorophore, 683/703 nm [Cy5.5-TP] M100435
Pyrene Internal Phosphoramidite intercalating fluorophore, 336/384 nm (lit) [SuperP-P] M830711
Pyrene-Super I 3'-Phosphoramidite intercalating fluorophore-Super I conjugate, 336/384 nm (lit) [SuperI-Py-3'-P] M830782
PBI3 MGB Polystyrene fluorescent minor groove binder, 358/473 nm [PBI3-SS] M100243
EB380-MGB Polystyrene fluorophore-MGB conjugate, 381/460 nm [EB380-MGB-SS] M100431
FAM-MGB Polystyrene fluorophore-MGB conjugate, 496/517 nm [FAM-MGB-SS] M100185
AP525-MGB Polystyrene fluorophore-MGB conjugate, 527/549 nm [AP525-MGB-SS] M100186
AP559-MGB Polystyrene fluorophore-MGB conjugate, 559/581 nm [AP559-MGB-SS] M100181
AP593-MGB Polystyrene fluorophore-MGB conjugate, 593/613 nm [AP593-MGB-SS] M100135
AP639-MGB Polystyrene fluorophore-MGB conjugate, 639/655 nm [AP639-MGB-SS] M100293
AP642-MGB Polystyrene fluorophore-MGB conjugate, 641/653 nm [AP642-MGB-SS] M100153
AP662-MGB Polystyrene fluorophore-MGB conjugate, 662/672 nm [AP662-MGB-SS] M100250
AP680-MGB Polystyrene fluorophore-MGB conjugate, 682/704 nm [AP680-MGB-SS] M100219
AP690-MGB Polystyrene fluorophore-MGB conjugate, 689/709 nm [AP690-MGB-SS] M100499
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Quenchers

name description abbreviation part number
Lite Quencher-380 Internal Phosphoramidite quencher, 388 nm [LQ-380-IP] M830786
Lite Quencher-380 Polystyrene quencher, 388 nm [LQ-380-SS] M100505
Lite Quencher-400 Internal Phosphoramidite quencher, 407 nm [LQ-400-IP] M830788
Lite Quencher-400 Polystyrene quencher, 407 nm [LQ-400-SS] M100507
Quencher-470 Internal Phosphoramidite quencher, 470 nm [Q-470-IP] M830565
Quencher-470 Polystyrene quencher, 470 nm [Q-470-SS] M100446
Eclipse Dark Quencher Phosphoramidite Eclipse Dark Quencher, 530 nm [EDQ-IP] M830028
Eclipse Dark Quencher Polystyrene Eclipse Dark Quencher, 530 nm [EDQ-SS] M100447
EDQ-Super I 3'-Phosphoramidite Super I conjugated with quencher, 530 nm, standard [SuperI-EDQ-3'-P] M830781
Quencher-575 Internal Phosphoramidite quencher, 575 nm [Q-575-IP] M830566
Quencher-575 Polystyrene quencher, 575 nm [Q-575-SS] M100448
Quencher-630 Internal Phosphoramidite quencher, 630 nm [Q-630-IP] M830567
Quencher-630 Polystyrene quencher, 630 nm [Q-630-SS] M100449
EDQ-MGB Polystyrene quencher-MGB conjugate, 530 nm [EDQ-MGB-SS] M130008
DSQ Polystyrene duplex stabilizing quencher, 549 nm [DSQ-SS] M100501
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Modified Nucleosides

name description abbreviation part number
FAM-dU 3'-Phosphoramidite uridine-fluorescein conjugate, 496/517 nm, standard [FAM-dU-3'-P] M830763
FAM-dU 5'-Phosphoramidite uridine-fluorescein conjugate, 496/517 nm, reverse [FAM-dU-5'-P] M830764
FAM-Super I 3'-Phosphoramidite Super I conjugated with fluorescein, 496/517 nm, standard [FAM-SuperI-3'-P] M830783
EDQ-Super I 3'-Phosphoramidite Super I conjugated with quencher, 530 nm, standard [SuperI-EDQ-3'-P] M830781
Super A 3'-Phosphoramidite stabilizing adenosine analogue, standard [SuperA-3'-P] M830249
Super A 5'-Phosphoramidite stabilizing adenosine analogue, reverse [SuperA-5'-P] M830250
Super G 3'-Phosphoramidite guanosine analogue, standard [SuperG-3'-P] M830006
Super G 5'-Phosphoramidite guanosine analogue, reverse [SuperG-5'-P] M830031
Super T 3'-Phosphoramidite stabilizing thymidine analogue, standard [SuperT-3'-P] M830040
Super T 5'-Phosphoramidite stabilizing thymidine analogue, reverse [SuperT-5'-P] M830041
Super I 3'-Phosphoramidite stabilizing inosine analogue, standard [SuperI-3'-P] M830193
Super I 5'-Phosphoramidite stabilizing inosine analogue, reverse [SuperI-5'-P] M830712
Super N 3'-Phosphoramidite universal base analogue, standard [SuperN-3'-P] M830162
Super N 5'-Phosphoramidite universal base analogue, reverse [SuperN-5'-P] M830165
Pyrene Internal Phosphoramidite intercalating fluorophore, 336/384 nm (lit) [SuperP-P] M830711
Pyrene-Super I 3'-Phosphoramidite intercalating fluorophore-Super I conjugate, 336/384 nm (lit) [SuperI-Py-3'-P] M830782
p-DNA T Phosphoramidite pyranosyl thymidine [p-DNA-T-P] M830779
p-DNA A Phosphoramidite pyranosyl adenosine [p-DNA-A-P] M830776
p-DNA C Phosphoramidite pyranosyl cytodine [p-DNA-C-P] M830777
p-DNA G Phosphoramidite pyranosyl guanosine [p-DNA-G-P] M830778
NNA T Phosphoramidite non-constrained thymidine [NNA-T-P] M830775
NNA A Phosphoramidite non-constrained adenosine [NNA-A-P] M830772
NNA C Phosphoramidite non-constrained cytodine [NNA-C-P] M830773
NNA G Phosphoramidite non-constrained guanosine [NNA-G-P] M830774
TFA-Aminopropyl-dU 3'-Phosphoramidite linker-uridine conjugate, standard [TFA-Amino-dU-3'-P] M830780
TFA-Aminopropyl-dU 5'-Phosphoramidite linker-uridine conjugate, reverse [TFA-Amino-dU-5'-P] M830715
TFA-Aminopropyl-Super G 3'-Phosphoramidite linker-Super G conjugate, standard [TFA-Amino-SuperG-3'-P] M830784
Deoxyinosine 3'-Phosphoramidite inosine, standard [I-3'-P] M100169
Deoxyinosine 5'-Phosphoramidite inosine, reverse [I-5'-P] TBD
2'-OMe-A-CE Phosphoramidite RNA adenosine, phosphoramidite [mA-P] M100545
2'-OMe-Ac-C-CE Phosphoramidite RNA cytodine, phosphoramidite [mC-P] M100544
2'-OMe-G-CE Phosphoramidite RNA guanosine, phosphoramidite [mG-P] M100546
2'-OMe-U-CE Phosphoramidite RNA thymidine, phosphoramidite [mU-P] M100543
2'-OMe-A-CE Solid Support RNA adenosine, solid support [mA-SS] M100539
2'-OMe-Ac-C-CE Solid Support RNA cytodine, solid support [mC-SS] M100542
2'-OMe-G-CE Solid Support RNA guanosine, solid support [mG-SS] M100540
2'-OMe-U-CE Solid Support RNA thymidine, solid support [mU-SS] M100541
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3’/5’ Mods

name description abbreviation part number
CDPI3 MGB Terminal Phosphoramidite CC-1065 minor groove binder [MGB-TP] M830252
CDPI3 MGB Polystyrene CC-1065 minor groove binder [MGB-SS] M100103
PBI3 MGB Polystyrene fluorescent minor groove binder, 358/473 nm [PBI3-SS] M100243
EDQ-MGB Polystyrene quencher-MGB conjugate, 530 nm [EDQ-MGB-SS] M130008
DSQ Polystyrene duplex stabilizing quencher, 549 nm [DSQ-SS] M100501
EB380-MGB Polystyrene fluorophore-MGB conjugate, 381/460 nm [EB380-MGB-SS] M100431
FAM-MGB Polystyrene fluorophore-MGB conjugate, 496/517 nm [FAM-MGB-SS] M100185
AP525-MGB Polystyrene fluorophore-MGB conjugate, 527/549 nm [AP525-MGB-SS] M100186
AP559-MGB Polystyrene fluorophore-MGB conjugate, 559/581 nm [AP559-MGB-SS] M100181
AP593-MGB Polystyrene fluorophore-MGB conjugate, 593/613 nm [AP593-MGB-SS] M100135
AP639-MGB Polystyrene fluorophore-MGB conjugate, 639/655 nm [AP639-MGB-SS] M100293
AP642-MGB Polystyrene fluorophore-MGB conjugate, 641/653 nm [AP642-MGB-SS] M100153
AP662-MGB Polystyrene fluorophore-MGB conjugate, 662/672 nm [AP662-MGB-SS] M100250
AP680-MGB Polystyrene fluorophore-MGB conjugate, 682/704 nm [AP680-MGB-SS] M100219
AP690-MGB Polystyrene fluorophore-MGB conjugate, 689/709 nm [AP690-MGB-SS] M100499
Fmoc-HEG-MGB Polystyrene MGB-linker [Fmoc-HEG-MGB-SS] M100508
×

Linkers

name description abbreviation part number
Super I 3'-Phosphoramidite stabilizing inosine analogue with amine, standard [SuperI-3'-P] M830193
Super I 5'-Phosphoramidite stabilizing inosine analogue with amine, reverse [SuperI-5'-P] M830712
Cholesterol Phosphoramidite lipophilic modification [Cholesterol-P] M830785
Cholesterol CPG lipophilic modification [Cholesterol-SS] M830761
Benzaldehyde Phosphoramidite Schiff base substrate, terminal [Benzaldehyde-P] M830547
DMT-Hexanol Diglycolate Polystyrene hexanol [Hexanol-SS] M100457
TFA-Aminopropyl-dU 3'-Phosphoramidite amine linker-uridine conjugate, standard [TFA-Amino-dU-3'-P] M830780
TFA-Aminopropyl-dU 5'-Phosphoramidite amine linker-uridine conjugate, reverse [TFA-Amino-dU-5'-P] M830715
TFA-Aminopropyl-Super G 3'-Phosphoramidite amine linker-Super G conjugate, standard [TFA-Amino-SuperG-3'-P] M830784
Fmoc-Hydroxyprolinol Phosphoramidite amine trifunctional cyclic linker, internal [Fmoc-HP-P] M830769
Fmoc-Hydroxyprolinol Polystyrene amine trifunctional cyclic linker [Fmoc-HP-SS] M100510
Fmoc-Aminohexanediol Polystyrene amine trifunctional aliphatic linker, internal [Fmoc-Amino-C6-SS] M100511
Fmoc-HEG Phosphoramidite amine trifunctional cyclic long linker, internal [Fmoc-HEG-P] M830767
Fmoc-HEG-MGB Polystyrene MGB-amine linker [Fmoc-HEG-MGB-SS] M100508
Spacer 18 Phosphoramidite (HEG) hexaethylene glycol linker, internal [Spacer-18-P] M100013
Biotin streptavidin-binding substrate [BiotinTEG-P] M160313
Phosphorylation Reagent II (Glen 10-1901) terminal phosphate [5Phos-TP] M130026

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Oligonucleotide Synthesis

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oligonucleotide labeling & synthesis guide

Products in the Reagent Catalog do not fall under the EN ISO 13485:2016 scope of “the design and manufacture of in vitro diagnostic PCR based primers, probes, reagents, calibrators, templates, controls, and test kits.”

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