Oligonucleotide Ordering Form

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Oligo Form User Instructions

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Oligo Description (required)

Part Number (see instructions)

Notes

Sequence (5' → 3’) (required) Maximum length is 45

Optional Services

Scale

200 nmol

1 µmol

Guaranteed nmol:

Probe minimum – 25 nmols
Primer minimum – 100 nmols

Purification

Formulation

Analysis

Quality Options

Research grade

CoA (Research grade)

cGMP (ISO 13485)

Please expect an additional 3 business days for a first-time ISO 13485:2016 order.

Oligo Analysis

Quote	$
Synthesizability
Estimated Minimum Yield (nmol)*
%GC Content
Total Bases
Modifications
Molecular Mass (Da)
Estimated E260 (M^-1cm^-1)
Estimated T_m (°C)*

Sequence Assembly

*Notes

Estimated minimum yield is not guaranteed.
T_m calculation is performed with the following conditions: 50 mM Na⁺,
3 mM Mg⁺⁺, 1 µM oligonucleotide (equimolar to complement).

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- The use of oligonucleotides modified with 3’-MGB-Quenchers and 5’-Fluorophores is limited to human IVD applications only.
- ELITechGroup MDx holds patents on MGB, DSQ, AquaPhluor dyes, Super Bases, and oligonucleotide applications. Research-scale oligonucleotides containing these technologies are available for use without a license for research applications. Licensing may be required for large-scale orders and non-research use. Contact ELITechGroup MDx for more information.

Fluorophores

name	description	abbreviation	part number
Epoch Blue 380 Internal Phosphoramidite	fluorophore, 381/460 nm	[EB380-IP]	M830192
Epoch Blue 380-HEG Phosphoramidite	fluorophore, 381/460 nm, long linker	[EB380-HEG-P]	M830727
Epoch Blue 380 Polystyrene	fluorophore, 381/460 nm	[EB380-SS]	M100441
6-FAM Phosphoramidite	fluorophore, 496/517 nm, short linker	[FAM-P]	M100011
FAM-HEG Phosphoramidite	fluorophore, 496/517 nm, long linker	[FAM-HEG-IP]	M830100
FAM-HEG Polystyrene	fluorophore, 496/517 nm, long linker	[FAM-HEG-SS]	M100442
FAM-dU 3'-Phosphoramidite	uridine-fluorescein conjugate, 496/517 nm, standard	[FAM-dU-3'-P]	M830763
FAM-dU 5'-Phosphoramidite	uridine-fluorescein conjugate, 496/517 nm, reverse	[FAM-dU-5'-P]	M830764
FAM-Super I 3'-Phosphoramidite	Super I conjugated with fluorescein, 496/517 nm, standard	[FAM-SuperI-3'-P]	M830783
Gig Harbor Green Polystyrene	fluorophore, 497/521 nm, short linker	[GG-SS]	M100433
AquaPhluor 525 Internal Phosphoramidite	fluorophore, 527/549 nm, short linker	[AP525-IP]	M830199
AquaPhluor 525 Terminal Phosphoramidite	fluorophore, 527/549 nm, short linker	[AP525-TP]	M820714
AquaPhluor 525-HEG Phosphoramidite	fluorophore, 527/549 nm, long linker	[AP525-HEG-P]	M100104
AquaPhluor 525 Polystyrene	fluorophore, 527/549 nm, short linker	[AP525-SS]	M100276
Yakima Yellow Phosphoramidite	fluorophore, 525/547 nm, short linker	[YY-P]	M830552
Yakima Yellow Polystyrene	fluorophore, 525/547 nm, short linker	[YY-SS]	M100443
AquaPhluor 530 Terminal Phosphoramidite	fluorophore, 531/552 nm, short linker	[AP530-TP]	M830803
AquaPhluor 559 Internal Phosphoramidite	fluorophore, 559/581 nm, long linker	[AP559-IP]	M830195
AquaPhluor 559 Terminal Phosphoramidite	fluorophore, 559/581 nm, short linker	[AP559-TP]	M830698
AquaPhluor 559 Polystyrene	fluorophore, 559/581 nm, short linker	[AP559-SS]	M100278
AquaPhluor 570 Terminal Phosphoramidite	fluorophore, 570/592 nm, short linker	[AP570-TP]	M830699
AquaPhluor 570 Internal Phosphoramidite	fluorophore, 570/592 nm, short linker	[AP570-IP]	M830675
AquaPhluor 570 Polystyrene	fluorophore, 570/592 nm, short linker	[AP570-SS]	M100444
Redmond Red Phosphoramidite	fluorophore, 580/594 nm, short linker	[RR-P]	M830035
Redmond Red Polystyrene	fluorophore, 580/594 nm, short linker	[RR-SS]	M100445
AquaPhluor 593 Terminal Phosphoramidite	fluorophore, 593/613 nm, short linker	[AP593-TP]	M830736
AquaPhluor 593 Polystyrene	fluorophore, 593/613 nm, short linker	[AP593-SS]	M100257
AquaPhluor 639 Terminal Phosphoramidite	fluorophore, 593/613 nm, short linker	[AP639-TP]	M830741
AquaPhluor 639 Polystyrene	fluorophore, 639/655 nm, short linker	[AP639-SS]	M100440
AquaPhluor 642 Polystyrene	fluorophore, 641/653 nm, short linker	[AP642-SS]	M100280
AquaPhluor 662 Polystyrene	fluorophore, 662/672 nm, short linker	[AP662-SS]	M100271
AquaPhluor 680 Polystyrene	fluorophore, 682/704 nm, short linker	[AP680-SS]	M100282
AquaPhluor 690 Terminal Phosphoramidite	fluorophore, 689/709 nm, short linker	[AP690-TP]	M830760
AquaPhluor 690 Polystyrene	fluorophore, 689/709 nm, short linker	[AP690-SS]	M100500
Quasar 705 Terminal Phosphoramidite	fluorophore, 690/705 nm	[QSR705-TP]	M100274
Cyanine 5.5 Terminal Phosphoramidite	fluorophore, 683/703 nm	[Cy5.5-TP]	M100435
Pyrene Internal Phosphoramidite	intercalating fluorophore, 336/384 nm (lit)	[SuperP-P]	M830711
Pyrene-Super I 3'-Phosphoramidite	intercalating fluorophore-Super I conjugate, 336/384 nm (lit)	[SuperI-Py-3'-P]	M830782
PBI3 MGB Polystyrene	fluorescent minor groove binder, 358/473 nm	[PBI3-SS]	M100243
EB380-MGB Polystyrene	fluorophore-MGB conjugate, 381/460 nm	[EB380-MGB-SS]	M100431
FAM-MGB Polystyrene	fluorophore-MGB conjugate, 496/517 nm	[FAM-MGB-SS]	M100185
AP525-MGB Polystyrene	fluorophore-MGB conjugate, 527/549 nm	[AP525-MGB-SS]	M100186
AP559-MGB Polystyrene	fluorophore-MGB conjugate, 559/581 nm	[AP559-MGB-SS]	M100181
AP593-MGB Polystyrene	fluorophore-MGB conjugate, 593/613 nm	[AP593-MGB-SS]	M100135
AP639-MGB Polystyrene	fluorophore-MGB conjugate, 639/655 nm	[AP639-MGB-SS]	M100293
AP642-MGB Polystyrene	fluorophore-MGB conjugate, 641/653 nm	[AP642-MGB-SS]	M100153
AP662-MGB Polystyrene	fluorophore-MGB conjugate, 662/672 nm	[AP662-MGB-SS]	M100250
AP680-MGB Polystyrene	fluorophore-MGB conjugate, 682/704 nm	[AP680-MGB-SS]	M100219
AP690-MGB Polystyrene	fluorophore-MGB conjugate, 689/709 nm	[AP690-MGB-SS]	M100499

Quenchers

name	description	abbreviation	part number
Lite Quencher-380 Internal Phosphoramidite	quencher, 388 nm	[LQ-380-IP]	M830786
Lite Quencher-380 Polystyrene	quencher, 388 nm	[LQ-380-SS]	M100505
Lite Quencher-400 Internal Phosphoramidite	quencher, 407 nm	[LQ-400-IP]	M830788
Lite Quencher-400 Polystyrene	quencher, 407 nm	[LQ-400-SS]	M100507
Quencher-470 Internal Phosphoramidite	quencher, 470 nm	[Q-470-IP]	M830565
Quencher-470 Polystyrene	quencher, 470 nm	[Q-470-SS]	M100446
Eclipse Dark Quencher Phosphoramidite	Eclipse Dark Quencher, 530 nm	[EDQ-IP]	M830028
Eclipse Dark Quencher Polystyrene	Eclipse Dark Quencher, 530 nm	[EDQ-SS]	M100447
EDQ-Super I 3'-Phosphoramidite	Super I conjugated with quencher, 530 nm, standard	[SuperI-EDQ-3'-P]	M830781
Quencher-575 Internal Phosphoramidite	quencher, 575 nm	[Q-575-IP]	M830566
Quencher-575 Polystyrene	quencher, 575 nm	[Q-575-SS]	M100448
Quencher-630 Internal Phosphoramidite	quencher, 630 nm	[Q-630-IP]	M830567
Quencher-630 Polystyrene	quencher, 630 nm	[Q-630-SS]	M100449
EDQ-MGB Polystyrene	quencher-MGB conjugate, 530 nm	[EDQ-MGB-SS]	M130008
DSQ Polystyrene	duplex stabilizing quencher, 549 nm	[DSQ-SS]	M100501

Modified Nucleosides

name	description	abbreviation	part number
FAM-dU 3'-Phosphoramidite	uridine-fluorescein conjugate, 496/517 nm, standard	[FAM-dU-3'-P]	M830763
FAM-dU 5'-Phosphoramidite	uridine-fluorescein conjugate, 496/517 nm, reverse	[FAM-dU-5'-P]	M830764
FAM-Super I 3'-Phosphoramidite	Super I conjugated with fluorescein, 496/517 nm, standard	[FAM-SuperI-3'-P]	M830783
EDQ-Super I 3'-Phosphoramidite	Super I conjugated with quencher, 530 nm, standard	[SuperI-EDQ-3'-P]	M830781
Super A 3'-Phosphoramidite	stabilizing adenosine analogue, standard	[SuperA-3'-P]	M830249
Super A 5'-Phosphoramidite	stabilizing adenosine analogue, reverse	[SuperA-5'-P]	M830250
Super G 3'-Phosphoramidite	guanosine analogue, standard	[SuperG-3'-P]	M830006
Super G 5'-Phosphoramidite	guanosine analogue, reverse	[SuperG-5'-P]	M830031
Super T 3'-Phosphoramidite	stabilizing thymidine analogue, standard	[SuperT-3'-P]	M830040
Super T 5'-Phosphoramidite	stabilizing thymidine analogue, reverse	[SuperT-5'-P]	M830041
Super I 3'-Phosphoramidite	stabilizing inosine analogue, standard	[SuperI-3'-P]	M830193
Super I 5'-Phosphoramidite	stabilizing inosine analogue, reverse	[SuperI-5'-P]	M830712
Super N 3'-Phosphoramidite	universal base analogue, standard	[SuperN-3'-P]	M830162
Super N 5'-Phosphoramidite	universal base analogue, reverse	[SuperN-5'-P]	M830165
Pyrene Internal Phosphoramidite	intercalating fluorophore, 336/384 nm (lit)	[SuperP-P]	M830711
Pyrene-Super I 3'-Phosphoramidite	intercalating fluorophore-Super I conjugate, 336/384 nm (lit)	[SuperI-Py-3'-P]	M830782
p-DNA T Phosphoramidite	pyranosyl thymidine	[p-DNA-T-P]	M830779
p-DNA A Phosphoramidite	pyranosyl adenosine	[p-DNA-A-P]	M830776
p-DNA C Phosphoramidite	pyranosyl cytodine	[p-DNA-C-P]	M830777
p-DNA G Phosphoramidite	pyranosyl guanosine	[p-DNA-G-P]	M830778
NNA T Phosphoramidite	non-constrained thymidine	[NNA-T-P]	M830775
NNA A Phosphoramidite	non-constrained adenosine	[NNA-A-P]	M830772
NNA C Phosphoramidite	non-constrained cytodine	[NNA-C-P]	M830773
NNA G Phosphoramidite	non-constrained guanosine	[NNA-G-P]	M830774
TFA-Aminopropyl-dU 3'-Phosphoramidite	linker-uridine conjugate, standard	[TFA-Amino-dU-3'-P]	M830780
TFA-Aminopropyl-dU 5'-Phosphoramidite	linker-uridine conjugate, reverse	[TFA-Amino-dU-5'-P]	M830715
TFA-Aminopropyl-Super G 3'-Phosphoramidite	linker-Super G conjugate, standard	[TFA-Amino-SuperG-3'-P]	M830784
Deoxyinosine 3'-Phosphoramidite	inosine, standard	[I-3'-P]	M100169
Deoxyinosine 5'-Phosphoramidite	inosine, reverse	[I-5'-P]	TBD
2'-OMe-A-CE Phosphoramidite	RNA adenosine, phosphoramidite	[mA-P]	M100545
2'-OMe-Ac-C-CE Phosphoramidite	RNA cytodine, phosphoramidite	[mC-P]	M100544
2'-OMe-G-CE Phosphoramidite	RNA guanosine, phosphoramidite	[mG-P]	M100546
2'-OMe-U-CE Phosphoramidite	RNA thymidine, phosphoramidite	[mU-P]	M100543
2'-OMe-A-CE Solid Support	RNA adenosine, solid support	[mA-SS]	M100539
2'-OMe-Ac-C-CE Solid Support	RNA cytodine, solid support	[mC-SS]	M100542
2'-OMe-G-CE Solid Support	RNA guanosine, solid support	[mG-SS]	M100540
2'-OMe-U-CE Solid Support	RNA thymidine, solid support	[mU-SS]	M100541

3’/5’ Mods

name	description	abbreviation	part number
CDPI3 MGB Terminal Phosphoramidite	CC-1065 minor groove binder	[MGB-TP]	M830252
CDPI3 MGB Polystyrene	CC-1065 minor groove binder	[MGB-SS]	M100103
PBI3 MGB Polystyrene	fluorescent minor groove binder, 358/473 nm	[PBI3-SS]	M100243
EDQ-MGB Polystyrene	quencher-MGB conjugate, 530 nm	[EDQ-MGB-SS]	M130008
DSQ Polystyrene	duplex stabilizing quencher, 549 nm	[DSQ-SS]	M100501
EB380-MGB Polystyrene	fluorophore-MGB conjugate, 381/460 nm	[EB380-MGB-SS]	M100431
FAM-MGB Polystyrene	fluorophore-MGB conjugate, 496/517 nm	[FAM-MGB-SS]	M100185
AP525-MGB Polystyrene	fluorophore-MGB conjugate, 527/549 nm	[AP525-MGB-SS]	M100186
AP559-MGB Polystyrene	fluorophore-MGB conjugate, 559/581 nm	[AP559-MGB-SS]	M100181
AP593-MGB Polystyrene	fluorophore-MGB conjugate, 593/613 nm	[AP593-MGB-SS]	M100135
AP639-MGB Polystyrene	fluorophore-MGB conjugate, 639/655 nm	[AP639-MGB-SS]	M100293
AP642-MGB Polystyrene	fluorophore-MGB conjugate, 641/653 nm	[AP642-MGB-SS]	M100153
AP662-MGB Polystyrene	fluorophore-MGB conjugate, 662/672 nm	[AP662-MGB-SS]	M100250
AP680-MGB Polystyrene	fluorophore-MGB conjugate, 682/704 nm	[AP680-MGB-SS]	M100219
AP690-MGB Polystyrene	fluorophore-MGB conjugate, 689/709 nm	[AP690-MGB-SS]	M100499
Fmoc-HEG-MGB Polystyrene	MGB-linker	[Fmoc-HEG-MGB-SS]	M100508

Linkers

name	description	abbreviation	part number
Super I 3'-Phosphoramidite	stabilizing inosine analogue with amine, standard	[SuperI-3'-P]	M830193
Super I 5'-Phosphoramidite	stabilizing inosine analogue with amine, reverse	[SuperI-5'-P]	M830712
Cholesterol Phosphoramidite	lipophilic modification	[Cholesterol-P]	M830785
Cholesterol CPG	lipophilic modification	[Cholesterol-SS]	M830761
Benzaldehyde Phosphoramidite	Schiff base substrate, terminal	[Benzaldehyde-P]	M830547
DMT-Hexanol Diglycolate Polystyrene	hexanol	[Hexanol-SS]	M100457
TFA-Aminopropyl-dU 3'-Phosphoramidite	amine linker-uridine conjugate, standard	[TFA-Amino-dU-3'-P]	M830780
TFA-Aminopropyl-dU 5'-Phosphoramidite	amine linker-uridine conjugate, reverse	[TFA-Amino-dU-5'-P]	M830715
TFA-Aminopropyl-Super G 3'-Phosphoramidite	amine linker-Super G conjugate, standard	[TFA-Amino-SuperG-3'-P]	M830784
Fmoc-Hydroxyprolinol Phosphoramidite	amine trifunctional cyclic linker, internal	[Fmoc-HP-P]	M830769
Fmoc-Hydroxyprolinol Polystyrene	amine trifunctional cyclic linker	[Fmoc-HP-SS]	M100510
Fmoc-Aminohexanediol Polystyrene	amine trifunctional aliphatic linker, internal	[Fmoc-Amino-C6-SS]	M100511
Fmoc-HEG Phosphoramidite	amine trifunctional cyclic long linker, internal	[Fmoc-HEG-P]	M830767
Fmoc-HEG-MGB Polystyrene	MGB-amine linker	[Fmoc-HEG-MGB-SS]	M100508
Spacer 18 Phosphoramidite (HEG)	hexaethylene glycol linker, internal	[Spacer-18-P]	M100013
Biotin	streptavidin-binding substrate	[BiotinTEG-P]	M160313
Phosphorylation Reagent II (Glen 10-1901)	terminal phosphate	[5Phos-TP]	M130026

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oligonucleotide labeling & synthesis guide

Products in the Reagent Catalog do not fall under the EN ISO 13485:2016 scope of “the design and manufacture of in vitro diagnostic PCR based primers, probes, reagents, calibrators, templates, controls, and test kits.”

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