Oligonucleotide Synthesis

Oligonucleotide manufacturing and development expertise

Since our inception in 1985, we have been developing fluorescence-based nucleic acid probe technologies that strongly rely on automated DNA synthesis. Our long-standing, in-house oligonucleotide synthesis expertise makes us industry leaders.

Over the years, we have introduced hundreds of innovative specialty phosphoramidites and solid supports including minor groove binders (MGB), unique modified nucleobases, fluorophores, and quenchers. We have developed and optimized the manufacturing and quality control of thousands of high complexity nucleic acid probes and PCR primers in the cGMP environment. We offer a vertically integrated system of production: all steps are performed in-house, starting from the development of specialty reagents to the synthesis of probes and primers and final diagnostic kit assembly.

We understand the oligonucleotide needs of end users because we are end users ourselves. Our proprietary reagents and techniques serve to provide labeled probes with the desired physicochemical properties and, additionally, to simplify the probes’ synthesis and purification. Our comprehensive catalog for nucleic acid labeling includes a wide range of fluorescent dyes, modified nucleobases, minor groove binders and quenchers in a variety of reagent forms. The modifications in our catalog can be considered building blocks for the researcher’s imagination.

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Custom oligonucleotide synthesis

DNA synthesis services

DNA synthesis services are essential for various research applications, including gene editing, gene silencing, and synthetic biology. EGMDx provides custom DNA synthesis services that are tailored to meet the specific needs of researchers and biotech companies. We use technology, brainpower, and quality control measures to ensure the purity and accuracy of our synthesized DNA products. Our team also provides modifications, labeling, and purification options upon request.

Oligonucleotide modifications

Oligonucleotide modifications are essential for various research applications, including gene expression analysis, aptamer development, and siRNA delivery. EGMDx offers a range of modification options, including fluorescent dyes and other labels, to customize oligonucleotides for specific research needs. We set a high bar for ourselves in performance to ensure purity and accuracy of our modified oligonucleotides. We provide custom designed oligonucleotide sequences with modifications upon request.

Purification and quality control

Oligonucleotide purification and quality control are critical steps to ensure excellent output of synthesized oligonucleotides. EGMDx provides oligonucleotide purification services, including HPLC to remove impurities and ensure purity of product. Our facility for manufacturing oligonucleotides is cGMP compliant and ISO 13485 certified.

An Experienced Oligonucleotide Manufacturing Company

• Proprietary chemistry products with nearly 40 years of experience and sector knowledge

• Extreme competency in producing complex, high quality oligonucleotides

• Versatile solutions for automated and manual oligonucleotide labeling

• Wide variety of high-purity products

• Specifications for all phosphoramidites and solid supports include functional testing

• Key methodologies: DNA synthesis, automated flash chromatography, NMR spectroscopy, mass spectrometry, and HPLC for quality control

• All oligonucleotide manufacturing and QC performed onsite in Bothell, WA

Reagent Catalog

2024 Pricelist

oligonucleotides ElLITechgroup MDx

INFECTIOUS DISEASE TEST PORTFOLIO

Real-Time PCR testing for laboratories

ELITechGroup MDx set an industry standard in real-time PCR with the introduction of minor groove binder (MGB) probe technology, featured in our MGB Alert® products. MGB probe technology has long been the gold standard for hybridization probes that allow for melt curve analysis. Adding to our legacy of innovation, we now offer DSQ Alert probe technology, an evolution in MGB probe chemistry. Our DSQ Alert reagents feature our newest proprietary duplex stabilizing quencher chemistry in hydrolysis probes, offering enhanced signal-to-noise fluorescence and DNA duplex stability. All test reagents may be used on ELITechGroup MDx proprietary instrument systems and on most open channel platforms. 

Our PCR offerings

High Throughput Reagents

Master Mixes

In Development

  • HT HDV
  • HT HSV 1&2/VZV
  • HT Legionella
  • HT Norovirus GI/II

*IVD/EUA
§Analyte Specific Reagent. Analytical and performance characteristics are not established.
For Research Use Only. Not for use in diagnostic procedures.

MOLECULAR DIAGNOSTICS SYSTEM

The ELITe BeGenius®

Extraction, amplification, results.

With automated extraction, amplification, and results interpretation, the ELITe BeGenius is a sample-to-result solution for your lab. A 3-hour turnaround time means up to 3 hours of walkaway time per run.

Bring more power and flexibility into your lab.

Open mode allows a customizable workflow. 24 PCR wells offer independent thermocycling capability. The ELITe BeGenius features:

  • Full automation with onboard extraction and real-time PCR
  • Total nucleic acid extraction of RNA and DNA
  • Open system capability
  • 24 PCR wells, each with independent thermocycling capability
  • Multiple tests from each eluate
  • Post amplification melt curve option
  • Qualitative and quantitative capable
  • Up to 72 sample results in an 8-hour shift
  • Onboard cooled storage of samples, eluates, and reagents
  • 6 optical channels with cross-talk customization
  • Automated results interpretation
  • LIS integration

About ELITechGroup

ELITechGroup Corporate

At a global corporate level, ELITechGroup is an integrated in-vitro diagnostics company that develops, manufactures and markets a wide range of diagnostic products and solutions, including reagents, instruments, and software. More than 650 employees make ELITechGroup a trusted partner in the IVD industry, serving hospitals and diagnostic laboratories in more than 100 countries through direct sales and third-party distribution.

To optimally serve customers in an ever-evolving IVD market, ELITechGroup is organized into four business units dedicated to specific IVD technologies and market segments: Molecular Diagnostics (MDx), Clinical Chemistry, Biomedical Systems, and Microbiology.

ELITechGroup in-vitro diagnostics company
ELITechGroup MDx - Molecular Diagnostics System Test Kits

ELITechGroup Molecular Diagnostics US

ELITechGroup MDx LLC is the company’s US Molecular Diagnostics entity, based in Bothell, WA. The US MDx group serves the needs of clinical and research laboratories alike, offering an extensive range of molecular diagnostics products.

The US product portfolio includes the fully automated sample-to-result ELITe BeGenius instrument system and a growing menu of infectious disease tests and reagents that feature the innovative MGB Alert and DSQ Alert probe chemistries.

Why ELITechGroup?

Extensive IP portfolio

Inventors of MGB probes

cGMP compliant, ISO13485 certified

High throughput oligonucleotide manufacturing

25,000 sq. ft. corporate office in Bothell, WA 

An Evolution of Real-Time PCR Chemistry

Our legacy of innovation is historic, and continues with DSQ chemistry, merging the original MGB probe and fluorescence quenchers first brought to you by ELITechGroup MDx in the 1990s.

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Reagents for Oligonucleotide Labeling

Oligonucleotide Synthesis

Oligo Order Form

Reagent Catalog

oligonucleotide labeling & synthesis guide

Products in the Reagent Catalog do not fall under the EN ISO 13485:2016 scope of “the design and manufacture of in vitro diagnostic PCR based primers, probes, reagents, calibrators, templates, controls, and test kits.”

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(833) 227-3400

21720 23rd Drive SE, Suite #150

Bothell, WA 98021

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