Enterovirus PCR

Enterovirus real-time PCR

Non-polio enterovirus infections often cause mild illness like the common cold. Some enterovirus infections, however, can have serious effects, especially in infants and people with weakened immune systems. Enterovirus infections can cause viral meningitis, viral encephalitis, acute flaccid paralysis, hand, foot and mouth disease, and other complications.1 Viral meningitis is of particular concern among children’s hospitals, with diagnostic testing for enterovirus in cerebrospinal fluid specimens by PCR recognized as the gold standard.2

Parechovirus real-time PCR

In the pediatric patient population, clinicians also frequently order parechovirus testing.3 In 2022 the CDC issued a health advisory stating that human parechovirus (PeV) should be considered in infants presenting with fever, sepsis-like syndrome, or neurologic illness (seizures, meningitis) without another known cause, and cautioned that severe illness can occur in children under 3 months.4

Primers and probes

ELITechGroup MDx offers their redesigned v2.0 enterovirus and parechovirus analyte specific reagents* (ASR). The ASRs are intended to be used in the development of real-time PCR assays and were reengineered for both performance and workflow functionality. The two sets of reagents are optimized for singleplex PCR efficiency at lower copy numbers and minimal cross reactivity when biplexed.

*Analyte Specific Reagent. Analytical and performance characteristics are not established.

1Centers for Disease Control and Prevention. (2020, August 8). Non-Polio Enterovirus. Centers for Disease Control and Prevention. Retrieved November 1, 2023, from https://www.cdc.gov/non-polio-enterovirus/index.html

2Centers for Disease Control and Prevention. (2020, August 8). Non-Polio Enterovirus. Centers for Disease Control and Prevention. Retrieved November 1, 2023, from https://www.cdc.gov/non-polio-enterovirus/index.html

3Olijve L, Jennings L, Walls T. Human Parechovirus: an Increasingly Recognized Cause of Sepsis-Like Illness in Young Infants. Clin Microbiol Rev. 2017;31(1):e00047-17. Published 2017 Nov 15. doi:10.1128/CMR.00047-17

4American Academy of Pediatrics. (2022, July 12). CDC issues advisory on parechovirus infections in infants. Retrieved November 1, 2023, from https://publications.aap.org/aapnews/news/20733/CDC-issues-advisory-on-parechovirus-infections.


Product Ordering Information





DSQ Alert™ Enterovirus v2.0 Primer Mix ASR

Enterovirus positive/negative, Ct value


DSQ Alert™ Enterovirus v2.0 Probe Mix ASR

Enterovirus positive/negative, Ct value


DSQ Alert™ Parechovirus v2.0 Primer Mix ASR

Parechovirus positive/negative, Ct value


DSQ Alert™ Parechovirus v2.0 Probe Mix ASR

Parechovirus positive/negative, Ct value

Enterovirus and parechovirus real-time PCR


Real-Time PCR testing for laboratories

ELITechGroup MDx set an industry standard in real-time PCR with the introduction of minor groove binder (MGB) probe technology, featured in our MGB Alert® products. MGB probe technology has long been the gold standard for hybridization probes that allow for melt curve analysis. Adding to our legacy of innovation, we now offer DSQ Alert probe technology, an evolution in MGB probe chemistry. Our DSQ Alert reagents feature our newest proprietary duplex stabilizing quencher chemistry in hydrolysis probes, offering enhanced signal-to-noise fluorescence and DNA duplex stability. All test reagents may be used on ELITechGroup MDx proprietary instrument systems and on most open channel platforms. 

Our PCR offerings

High Throughput Reagents

Master Mixes

In Development

  • HT HDV
  • HT HSV 1&2/VZV
  • HT Legionella
  • HT Norovirus GI/II

§Analyte Specific Reagent. Analytical and performance characteristics are not established.
For Research Use Only. Not for use in diagnostic procedures.


The ELITe BeGenius®

Extraction, amplification, results.

With automated extraction, amplification, and results interpretation, the ELITe BeGenius is a sample-to-result solution for your lab. A 3-hour turnaround time means up to 3 hours of walkaway time per run.

Bring more power and flexibility into your lab.

Open mode allows a customizable workflow. 24 PCR wells offer independent thermocycling capability. The ELITe BeGenius features:

  • Full automation with onboard extraction and real-time PCR
  • Total nucleic acid extraction of RNA and DNA
  • Open system capability
  • 24 PCR wells, each with independent thermocycling capability
  • Multiple tests from each eluate
  • Post amplification melt curve option
  • Qualitative and quantitative capable
  • Up to 72 sample results in an 8-hour shift
  • Onboard cooled storage of samples, eluates, and reagents
  • 6 optical channels with cross-talk customization
  • Automated results interpretation
  • LIS integration

About ELITechGroup

ELITechGroup Corporate

At a global corporate level, ELITechGroup is an integrated in-vitro diagnostics company that develops, manufactures and markets a wide range of diagnostic products and solutions, including reagents, instruments, and software. More than 650 employees make ELITechGroup a trusted partner in the IVD industry, serving hospitals and diagnostic laboratories in more than 100 countries through direct sales and third-party distribution.

To optimally serve customers in an ever-evolving IVD market, ELITechGroup is organized into four business units dedicated to specific IVD technologies and market segments: Molecular Diagnostics (MDx), Clinical Chemistry, Biomedical Systems, and Microbiology.

ELITechGroup in-vitro diagnostics company
ELITechGroup MDx - Molecular Diagnostics System Test Kits

ELITechGroup Molecular Diagnostics US

ELITechGroup MDx LLC is the company’s US Molecular Diagnostics entity, based in Bothell, WA. The US MDx group serves the needs of clinical and research laboratories alike, offering an extensive range of molecular diagnostics products.

The US product portfolio includes the fully automated sample-to-result ELITe BeGenius instrument system and a growing menu of infectious disease tests and reagents that feature the innovative MGB Alert and DSQ Alert probe chemistries.

Why ELITechGroup?

Extensive IP portfolio

Inventors of MGB probes

cGMP compliant, ISO13485 certified

High throughput oligonucleotide manufacturing

25,000 sq. ft. corporate office in Bothell, WA 

An Evolution of Real-Time PCR Chemistry

Our legacy of innovation is historic, and continues with DSQ chemistry, merging the original MGB probe and fluorescence quenchers first brought to you by ELITechGroup MDx in the 1990s.

Contact us





Contact us

Product Portfolio

Automated PCR system

Candida auris PCR

High throughput PCR

Vector-borne PCR

Social Media

(833) 227-3400

21720 23rd Drive SE, Suite #150

Bothell, WA 98021

Terms & Conditions | Privacy