Malaria PCR Test

The MGB Alert Malaria Typer v2.0 RUO Detection Reagent is a multiplex real-time PCR reagent designed withMGB Pleiades® hybridization probe chemistry to detect and distinguish DNA from Plasmodium falciparum, P.vivax, P. ovale, and P. malariae or knowlesi. To use this product effectively, thermal cycler parameters must include PCR thermal cycling with five color fluorescence detection. The reagent contains primers and probes specific to the 18S rRNA gene of Plasmodium species and a primer set and probe specific to an internal control (IC, sold separately). Probes are labeled with a fluorophore, a minor groove binder (MGB), and an Eclipse® Dark Quencher (EDQ). The Pleiades hybridization probe chemistry in this product is unique. The fluorescence of unhybridized probes is quenched by a 5’ MGB and a 3’ EDQ. During each cycle of PCR, the primers and probes anneal to their DNA target template, if present, and a new DNA strand is synthesized from the primers by a polymerase. Hybridization of the probe to its target DNA separates the fluorophore from its quencher and MGB, allowing fluorescence emission. As the polymerase encounters the Pleiades probe annealed to the DNA template, the MGB on the 5’ end of the probe blocks any 5’ → 3’exonuclease activity of the polymerase, and the probe is
displaced as DNA is synthesized. The PCR cycles result in exponential amplification of target DNA and, therefore, fluorescence levels. An optional dissociation stage results in exponential decrease of fluorescence levels, i.e., a melt curve, each characterized by a melt temperature (Tm).

CATALOG #	PRODUCT NAME	REPORTABLE RESULT
M400874	MGB Alert Malaria Typer v2.0 RUO Detection Reagent	Plasmodium falciparum, P. vivax, P. ovale, P. malariae/knowlesi

^§Analyte Specific Reagent. Analytical and performance characteristics are not established.